Fix 16-bit MS-DOS Subsystem Error Massage

This addition you get further information to troubleshoot error "16-bit MS-DOS Subsystem" error message when you run an MS-DOS or 16-Bit Windows program in Windows Server 2003 or Windows 2000 Server"

If you try to run an MS-DOS-based or a 16-bit Windows-based command on a Microsoft Windows Server 2003-based or a Microsoft Windows 2000 Server-based computer, you may receive one of the following error messages:

16-bit MS-DOS Subsystem
path to the program you are trying to start or install
C:\WINNT\system32\config.nt The system file is not suitable for running MS-DOS and Microsoft Windows applications. Choose 'Close' to terminate the application.

16-bit MS-DOS Subsystem
path to the program you are trying to run
config.nt The system file is not suitable for running MS-DOS and Microsoft Windows applications. Choose 'Close' to terminate the application.


16-bit MS-DOS Subsystem
path to the program you are trying to run
C:\WINNT\system32\autoexec.nt The system file is not suitable for running MS-DOS and Microsoft Windows applications. Choose 'Close' to terminate the application.


This issue can occur if any one of the following files are missing, damaged, or not located in the %systemroot%\System32 folder:

Autoexec.nt
Command.com
Config.nt

This post provide step by step Computer repair and troubleshooting tips to fix this issue

Follow the Steps

1. Insert the Windows Server 2003 CD or the Windows 2000 Server CD into the CD drive.
2. Click Start, click Run, type cmd, and then click OK.
3. Type the following commands, and press ENTER after each command:
expand D:\i386\config.nt_ %systemroot%\system32\config.nt

expand D:\i386\autoexec.nt_ %systemroot%\system32\autoexec.nt

expand D:\i386\command.co_ %systemroot%\system32\command.com

exit
Note The description of this procedure assumes that your CD drive letter is D. If your CD drive letter is not D, substitute the correct drive letter.
4. Start or install the program. If the issue is resolved, do not complete the remaining steps. If the issue is not resolved, go to the next step.
5. Start Notepad, and then type the following commands.

Note The Command.com file is not edited or created in the following process. Because of this, you may have to expand the file from your CD-ROM. See step 16 for instructions on how to do this.

dos=high, umb
device=%SYSTEMROOT%\system32\himem.sys
files=40

6. On the File menu, click Save As.
7. In the File Name box, type Config.nt, and then click Save. Close the Config.nt file.
8. On the File menu, click New.
9. In the new blank document, type the following entries.

@echo off
lh %SYSTEMROOT%\system32\mscdexnt.exe
lh %SYSTEMROOT%\system32\redir
lh %SYSTEMROOT%\system32\dosx
SET BLASTER=A220 I5 D1 P330 T3

10. On the File menu, click Save As.
11. In the File Name box, type Autoexec.nt, and then click Save. Close the Autoexec.nt file.
12. Start Windows Explorer. Locate the Config.nt file, right-click the Config.nt file, and then click Copy.
13. Right-click the %systemroot%\System32 folder, and then click Paste.
14. Locate the Autoexec.nt file, right-click the Autoexec.nt file, and then click Copy.
15. Right-click the %systemroot%\System32 folder, and then click Paste.
16. Expand the Command.com file from the Windows Server 2003 or the Windows 2000 Server CD-ROM. To do this, follow these steps:
1. Click Start, click Run, type cmd, and then click OK.
2.

expand cd-rom:\i386\command.co_ drive_letter:\system_root\system32\command.com

exit

17. Verify the existence of the folder to which the TEMP and TMP user environment variables are mapped. To do this, follow these steps:
1. Click Start, click Run, type sysdm.cpl in the Open box, and then click OK.
2. In the System Properties dialog box, click the Advanced tab, and then click Environment Variables.
3. In the User variables for UserName section, locate the TEMP and TMP variables. They are listed in the Variable column.
4. For the TEMP and TMP variables, note the corresponding folder that is listed in the Value column.
5. Start Windows Explorer. To do this, click Start, click Run, type explorer in the Open box, and then click OK.
6. Locate the folder that is specified for the TEMP and TMP variables. Make sure that this folder exists.

Note To locate the folder, you may have to turn on the feature that shows hidden files and folders. To do this, follow these steps:
1. On the Tools menu, click Folder Options, and then click the View tab.
2. Under Hidden files and folders, click Show hidden files and folders, and then click OK.
18. Restart your computer.